MUMBAI, India, Nov. 21 -- Intellectual Property India has published a patent application (202517097994 A) filed by Cytiva Bioprocess R&D AB, Uppsala, Sweden, on Oct. 10, for 'a chromatography material, use thereof, and a method for separating adeno-associated capsids.'
Inventor(s) include Maloisel, Jean-Luc; Hagner Mcwhirter, Asa; and Nemeth, Brigitta.
The application for the patent was published on Nov. 21, under issue no. 47/2025.
According to the abstract released by the Intellectual Property India: "The present disclosure is directed to a chromatography material comprising a support material in the form of a convection-based membranous structure comprising nanofibres, wherein the support material is functionalised with an anion exchange ligand at a ligand density of 300 mol/mL, wherein the chromatography material comprises a linker connecting the ligand to the support material, the linker comprising a linear backbone having a length of 2-16 atoms. Also provided is a chromatography device comprising a holder comprising the chromatography material as disclosed herein. Additionally, the present disclosure is directed to use of the herein disclosed chromatography material or chromatography device for separating adeno-associated virus capsids fully packaged with genetic material from adeno-associated virus capsids not fully packaged with genetic material. Further, the present disclosure provides a method for separating adeno-associated virus capsids fully packaged with genetic material from adeno-associated virus capsids not fully packaged with genetic material, the method comprising the following steps: (a) adding a liquid sample comprising adeno-associated virus capsids to the chromatography material as disclosed herein, wherein the liquid sample comprises adeno-associated virus capsids of a purity of at least 90% and of a concentration of at least 1012 adeno-associated virus capsids/ml, of which at least 10% of the adeno-associated virus capsids are adeno-associated virus capsids fully packaged with genetic material; (b) eluting the adeno-associated virus capsids fully packaged with genetic material from the chromatography material; wherein the adeno-associated virus capsids eluted in step (b) are eluted into at least one eluate fraction, which eluate fraction comprises at least 50% of the fully packaged adeno-associated virus capsids present in the liquid sample added in step (a), and wherein at least 60% of the adeno-associated virus capsids eluted in step (b) are fully packaged with genetic material."
The patent application was internationally filed on Apr. 08, 2024, under International application No.PCT/EP2024/059424.
Disclaimer: Curated by HT Syndication.
