MUMBAI, India, June 6 -- Intellectual Property India has published a patent application (202321074743 A) filed by Embio Limited, Maharashtra, on Dec. 2, 2023, for 'a process of preparation of formaldehyde tolerant transaminases for the transamination of 4-hydroxy-2-butanone.'
Inventor(s) include Dr. Arumugam Govind Swaminathan.
The application for the patent was published on June 6, under issue no. 23/2025.
According to the abstract released by the Intellectual Property India: "A process of preparation of formaldehyde tolerant transaminases for the transamination of 4-hydroxy-2-butanone [0047] The present invention discloses a process of preparation of formaldehyde tolerant transaminases and their use for the transamination of 4-hydroxy-2-butanone. The process of preparation comprises steps of retrieval of R-selective transaminases from databases, crystallographic analysis and homology modelling of the enzymes, conformational exploration and stability analysis of the enzymes, golden gate cloning of the enzyme in E. coli host system, induction of gene expression in the cloned E. coli cells, purification of expressed protein by affinity chromatography and evaluation of enzyme stability, evaluation of yield and recycling and purification of the substrate. The present invention discloses amino acid sequences of R-selective transaminases numbered SEQ ID NO.: 1,2,3,4. The in silico active site modification aids in improvement of formaldehyde tolerance. The process yields high percentage conversion by transaminases and is cost-effective and ecofriendly."
Disclaimer: Curated by HT Syndication.
