MUMBAI, India, March 13 -- Intellectual Property India has published a patent application (202611009792 A) filed by Ashoka Institute Of Technology And Management; Mr. Ankit Arya; Ms. Hema Yadav; Ms. Nidhi Singh; Mr. Raunak Singh; Ms. Deepika Varma; Er. Arpita Singh; Er. Shiny Jaiswal; and Dr. Sarika Shrivastava, Varanasi, Uttar Pradesh, on Jan. 30, for 'integrated method and system for isolation, characterization, and computational validation of amylase producing microorganisms.'

Inventor(s) include Mr. Ankit Arya; Ms. Hema Yadav; Ms. Nidhi Singh; Mr. Raunak Singh; Ms. Deepika Varma; Er. Arpita Singh; Er. Shiny Jaiswal; and Dr. Sarika Shrivastava.

The application for the patent was published on March 13, under issue no. 11/2026.

According to the abstract released by the Intellectual Property India: "The invention relates to an integrated method and system for screening, characterization, and validation of amylase-producing microorganisms from natural aquatic sources. Water samples collected from river environments are subjected to serial dilution and microbial isolation, followed by screening on starch agar to identify microorganisms exhibiting starch hydrolysis. Selected isolates are characterized using morphological and biochemical analysis and are cultured in a starch-supplemented growth medium to produce extracellular amylase. Enzyme activity is determined using a reducing-sugar based colorimetric assay with spectrophotometric measurement. Microorganisms confirmed to exhibit measurable amylase activity are subsequently subjected to computational validation comprising sequence retrieval, three-dimensional structural modeling of the amylase enzyme, and molecular docking with starch-derived substrates. Experimental enzyme activity data are correlated with computational interaction parameters to generate validated analytical outputs. The method enables reproducible linkage between laboratory-based enzymatic assessment and computational analysis within a coordinated workflow suitable for enzyme research and industrial screening applications."

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