MUMBAI, India, July 25 -- Intellectual Property India has published a patent application (202517067066 A) filed by Basf Agricultural Solutions US Llc, Research Triangle Park, U.S.A., on July 14, for 'single-cell methods for the discovery of disease resistance leads.'
Inventor(s) include De Vleesschauwer, David; and Denolf, Peter.
The application for the patent was published on July 25, under issue no. 30/2025.
According to the abstract released by the Intellectual Property India: "The present invention relates generally to the field of molecular biology and concerns a method for high-throughput screening and characterization of candidate plant pathogen effector gene(s) and/or candidate plant disease resistance gene(s) in individual plant cells, encompassing (i) the introduction into a plant protoplast of one or more expression cassette(s) comprising at least one candidate plant pathogen effector gene(s) and/or at least one candidate plant disease resistance gene(s), (ii) transient expression of the candidate plant pathogen effector gene(s) and/or candidate plant disease resistance gene(s), (iii) measurent of reactive oxygen species (ROS) produced by the protoplast, followed by (iv) isolation of the plant protoplast producing ROS and (v) identification of the introduced at least one candidate plant pathogen effector gene(s) and/or at least one candidate plant disease resistance gene(s). Alternatively, the protoplast is capable of expressing a screenable marker, allowing measurement of modulated screenable marker expression as a proxy for HR activation."
The patent application was internationally filed on Dec. 08, 2023, under International application No.PCT/EP2023/084852.
Disclaimer: Curated by HT Syndication.
