MUMBAI, India, Aug. 10 -- Intellectual Property India has published a patent application (202641087038 A) filed by Thanissha S K; Yogitha S J; Dhaheera M; Yogavaradhan G V; and Dr. J Bindhu on July 16, 2026, for From Sea To Cell: Sargassum Wighth Biomolecules As Next-Generation Natural Cryoprotectants For Rauvolfia Serpentina Cells.
Inventors include Thanissha S K; Yogitha S J; Dhaheera M; Yogavaradhan G V; and Dr. J Bindhu.
The application for the patent was published on July 31, 2026, under issue no. 31/2026.
Abstract: A hetrart The present invention relates to a novel, eco-friendly cryoprotective formulation developed from bioactive biomolecules extracted from the marine brown seaweed Sargassum wightii for the cryopreservation of Rauvoffla serpentina cells. The invention utilizes naturally occurring polysaccharides, including alginate, fucoidan, and latninarin, together with polyphenols. proteins, amino acids, and antioxidant compounds as natural cryoprotective agents. These biomolecules reduce freezing-induced cellular damage by minimizing intracellular ice crystal formation, stabilizing cell membranes, maintaining osmotic balance, and scavenging reactive oxygen species (ROS). The extracted biomolecules are characterized using FTIR, HPLC, UV-Visible spectroscopy, GC-MS, and other analytical techniques. Their cryoprotective efficacy is evaluated by assessing post-thaw cell viability, membrane integrity, oxidative stress, and regeneration of Rauvo/fra serpentina cells following cryogenic storage. Compared with conventional cryoprotectants such as DMSO and glycerol, the proposed formulation offers lower cytotoxicity, improved cell recovery, and enhanced preservation efficiency. The invention provides a sustainable, biodegradable, and biocompatible cryoprotectant suitable for plant cell cryobanking, conservation of endangered medicinal plants, tissue culture repositories, pharmaceutical biotechnology, and plant genetic resource preservation. Abstract The present invention relates to a natural cryoprotective formulation developed from bioactive biomolecules extracted from the marine brown seaweed Sargassum wightil for the cryopreservation of Rauvolfia sopentina cells. The formulation utilizes naturally occurring polysaccharides, polyphenols, proteins, amino acids, and antioxidants to protect cells from freezing-induced damage. These biomolecules reduce intracellular ice crystal formation, stabilize cell membranes, maintain osmotic balance, and minimize oxidative stress during cryogenic storage. The extracted biomolecules are characterized using UV—Visible Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Performance Liquid Chromatography (HPLC), Gas Chromatography—Mass Spectrometry (GC—MS), Scanning Electron Microscopy (SEM), and Zeta Potential Analysis to determine their chemical composition, morphology, and stability. The cryoprotective efficacy is evaluated through post-thaw cell viability, membrane integrity, oxidative stress, and regeneration assays. Compared with conventional cryoprotectants such as DMSO and glycerol, the proposed formulation offers lower cytotoxicity and improved post-thaw recovery. The invention provides a sustainable, biodegradable, and biocompatible alternative for medicinal plant cryopreservation with potential applications in plant tissue culture, germplasm conservation, pharmaceutical biotechnology, and long-term preservation of valuable plant genetic resources.
Disclaimer: Curated by HT Syndication.
