MUMBAI, India, June 24 -- Intellectual Property India has published a patent application (202411090985 A) filed by Amity University on November 22, 2024, for Method For Biodiesel Production From Psychrophilic Chlorella Vulgaris.

Inventors include Richa Pahariya; Abhishek Chauhan; and Tanu Jindal.

The application for the patent was published on June 12, 2026, under issue no. 24/2026.

Abstract: The present invention discloses a method for producing biodiesel from a psychrophilic microalgal strain Chlorella vulgaris (Chlorella sp. BDUGARC11071) comprising isolation and cultivation in BG11 (psychrophilic ~15 ± 2 °C or mesophilic ~25 ± 2 °C) under ~2500 lux and 18:6 light:dark; supplementation with laboratory-synthesized nanoparticles selected from Fe2O3, ZnO and ZrO2 (preferably 10 mg·L?¹) to enhance lipid accumulation; harvesting by centrifugation, washing and drying at ~60 °C; cell disruption (e.g., microwave 540 W, 2 min) and lipid extraction (Bligh & Dyer or chloroform:methanol system); and conversion of extracted lipids to fatty acid methyl esters (FAME) by methanol–base transesterification. Produced biodiesel and intermediates are characterizable by XRD, SEM-EDX, FTIR and GC-MS. The method affords increased lipid yield and improved FAME profile.

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