MUMBAI, India, Nov. 2 -- Intellectual Property India has published a patent application (202617093143 A) filed by Pegasus Biotech Incorporated on July 31, 2026, for Plasmid And Method For Production Of Dna Vaccines And Dna Products.
Inventors include Slaine, Patrick; Wilson, Daniel; Wilts, Jonathan; Mackenzie, Oliver; and Mena, Yimy.
The application for the patent was published on October 30, 2026, under issue no. 44/2026.
Abstract: A method for producing replicating DNA molecules is described, producing DNA products, such as vaccines or biotherapeutics. A parental DNA template comprises a sequence of interest, and a modified Col E1 origin lacking RNA I inhibition, having an inducible switch for recombinase translation control. The method permits control of plasmid DNA production during host fermentation by controlling relocation of a plasmid RNA II promoter to upstream of the RNA II primer template sequence through recombination, where RNA II initiates plasmid replication. The bacterial origin of a linearized plasmid is stably inserted into the host chromosome by separating the origin's promoter from the RNA II primer template sequence, allowing antibiotic-free fermentation while the plasmid is hidden within the chromosome. The excision of the targeted plasmid DNA is induced to control recombination, forming a plasmid with a functional bacterial origin lacking negative regulation, driving run-away amplification and high plasmid DNA production.
Disclaimer: Curated by HT Syndication.
