MUMBAI, India, Oct. 26 -- Intellectual Property India has published a patent application (202511123005 A) filed by Deen Dayal Upadhyaya Gorakhpur University on December 05, 2025, for Rapid Chromogenic Culture Medium Comprising L-Glutamic Acid And Bromocresol Purple For Enhanced Isolation And Screening Of Phosphate Solubilizing Bacteria.

Inventors include Priyanka Bharti; and Sarad Kumar Mishra.

The application for the patent was published on October 23, 2026, under issue no. 43/2026.

Abstract: The present invention relates to a rapid chromogenic culture medium comprising L-glutamic acid and bromocresol purple for enhanced isolation and screening of phosphate solubilizing bacteria (PSB). The medium utilizes a dual carbon system (molasses and starch), hybrid phosphate sources (TCP and rock phosphate), and optimized mineral composition for supporting fastidious strains. The L-glutamic acid acts as an organic acid precursor, accelerating phosphate solubilization, while bromocresol purple provides a visible color shift (purple to yellow) indicating solubilization. The medium enables rapid (18–24 hours) and cost-effective detection of PSB compared to conventional PVK and NBRIP media.

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