MUMBAI, India, Aug. 10 -- Intellectual Property India has published a patent application (202511002407 A) filed by Indian Council Of Agricultural Research on January 10, 2025, for Spore Based Paper Strip Sensor For Detection Of Aflatoxin B1 In Food And Feed.
Inventors include Dr. Priya Kalyan; Mr. Srishti Patel; Dr. Naresh Kumar; Dr. Raghu H V; and Dr. Rajan Sharma.
The application for the patent was published on July 31, 2026, under issue no. 31/2026.
Abstract: A two-stage enzyme-substrate based paper strip sensor has been developed for the rapid detection of Aflatoxin B1 (AFB1), leveraging Bacillus stearothermophilus- based spores and a-glucosidase as the marker enzyme. Screening of six enzymes identified a-glucosidase as the most sensitive, producing a distinct color change on functionalized paper strips within 30 minutes. Spore-based enzyme activity was optimized by adjusting spore concentration, substrate volume, incubation temperature, and reaction time, with 65°C and 30 minutes identified as optimal conditions. The assay demonstrated a limit of detection (LOD) of 10 ppb for AFB1, meeting regulatory thresholds for feed and food safety. Paper strips, fabricated using Whatman Grade 3 filter paper, exhibited reliable enzyme-substrate reactions, validated through extraction efficiency, inhibition assays, and chromogenic detection. Validation using ELISA and HPLC-FD confirmed the assay's accuracy, with results aligning closely with established analytical methods. Field trials on 120 feed and food samples demonstrated the assay's specificity, detecting AFB1 without cross- reactivity to other contaminants such as pesticides, heavy metals, and antibiotics. This novel paper strip biosensor provides a rapid, cost-effective, and field-deployable method for AFBI detection in diverse matrices, ensuring compliarice with safety standards and supporting efforts to mitigate aflatoxin-related risks in animal feed and food products.
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